Mouse Plexin A1/PLXNA1 ELISA Kit PicoKine®

Mouse Plexin A1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Plxna1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Plexin-A1 is a protein that in humans is encoded by the PLXNA1 gene. This gene is mapped to 6; 6 D1. Plexin A1 has been shown to interact with AKT1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 417 452 485 454 24.48 5.3% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 3 8962 10130 10185 9716 9748 488.80 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 462 417 452 485 454 24.48 5.3% Sample 1 462 417 452 485 454 24.48 5.3% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 2 2424 2827 2492 2766 2627 172.30 6.5% Sample 3 8962 10130 10185 9716 9748 488.80 5.0% Sample 3 8962 10130 10185 9716 9748 488.80 5.0%

Synonyms

Plexin-A1; Plex 1; Plexin-1; Plxna1; Kiaa4053

Gene Name

Plxna1

Gene ID

18844

UniProt

P70206

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S28-P1242

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Ubiquitous.

Applications

ELISA

Field of Research

Axonal Guidance Proteins, Growth and Development, More Gpcr, Neurogenesis, Neurology Process, Neuroscience, Neurotransmission, Receptors / Channels

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

0.312 ng/mL - 20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 462 2424 8962 500 2463 9301 Mean (pg/ml) 462 2424 8962 500 2463 9301 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 Standard deviation 21.71 123.62 412.25 27.00 155.17 697.58 CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5% CV (%) 4.7% 5.1% 4.6% 5.4% 6.3% 7.5%

Sensitivity

<0.1 ng/mL

Function

Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex compos

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Plexin A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin A1

Subcellular Location

Cell membrane, Single-pass type I membrane protein

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245 O.D. 0.067 0.154 0.192 0.272 0.492 0.732 1.391 2.245

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.