Human MANF ELISA Kit PicoKine®

Human MANF ELISA Kit PicoKine® (96 Tests) . Quantitate Human MANF in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 148 167 169 160 8.51 5.3% Sample 2 733 654 771 713 717 42.29 5.8% Sample 3 2725 2678 2428 2740 2642 126.07 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 156 148 167 169 160 8.51 5.3% Sample 1 156 148 167 169 160 8.51 5.3% Sample 2 733 654 771 713 717 42.29 5.8% Sample 2 733 654 771 713 717 42.29 5.8% Sample 3 2725 2678 2428 2740 2642 126.07 4.7% Sample 3 2725 2678 2428 2740 2642 126.07 4.7%

Synonyms

Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP

Gene Name

MANF

Gene ID

7873

UniProt

P55145

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L25-L182

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Amino Acids, Cancer, Cell Cycle, Cell Division, Neuroscience, Neurotransmitter

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

93.6 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 156 733 2725 166 748 2871 Mean (pg/ml) 156 733 2725 166 748 2871 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 Standard deviation 9.67 47.65 125.35 8.47 55.35 195.23 CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8% CV (%) 6.2% 6.5% 4.6% 5.1% 7.4% 6.8%

Sensitivity

<15 pg/mL

Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents i

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human MANF

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Mesencephalic astrocyte derived neurotrophic factor

Subcellular Location

Endoplasmic reticulum lumen, Secreted, Sarcoplasmic reticulum lumen

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229 O.D. 0.051 0.089 0.129 0.242 0.459 0.854 1.64 2.229

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.