Rat JAM-C ELISA Kit PicoKine®

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 8q13. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 35 34 32 2.06 6.4% Sample 2 159 171 172 173 168 5.67 3.3% Sample 3 382 367 365 370 371 6.59 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 35 34 32 2.06 6.4% Sample 1 30 31 35 34 32 2.06 6.4% Sample 2 159 171 172 173 168 5.67 3.3% Sample 2 159 171 172 173 168 5.67 3.3% Sample 3 382 367 365 370 371 6.59 1.7% Sample 3 382 367 365 370 371 6.59 1.7%

Atherosclerosis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Platelets, Signal Transduction, Tags & Cell Markers, Vascular Inflammation, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.