Rat JAM-C ELISA Kit PicoKine®

Rat JAM-C ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Jam3 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 8q13. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 35 34 32 2.06 6.4% Sample 2 159 171 172 173 168 5.67 3.3% Sample 3 382 367 365 370 371 6.59 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 30 31 35 34 32 2.06 6.4% Sample 1 30 31 35 34 32 2.06 6.4% Sample 2 159 171 172 173 168 5.67 3.3% Sample 2 159 171 172 173 168 5.67 3.3% Sample 3 382 367 365 370 371 6.59 1.7% Sample 3 382 367 365 370 371 6.59 1.7%

Synonyms

Junctional adhesion molecule C; JAM-C; Junctional adhesion molecule 3; JAM-3; Jam3

Gene Name

Jam3

Gene ID

315509

UniProt

Q68FQ2

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V32-N241

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Applications

ELISA

Field of Research

Atherosclerosis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Platelets, Signal Transduction, Tags & Cell Markers, Vascular Inflammation, Vasculature

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 30 159 382 31 163 472 Mean (pg/ml) 30 159 382 31 163 472 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 Standard deviation 1.29 7.31 21.77 1.92 10.27 34.93 CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4% CV (%) 4.3% 4.6% 5.7% 6.2% 6.3% 7.4%

Sensitivity

<5 pg/mL

Function

May participate in cell-cell adhesion distinct from tight junctions.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat JAM-C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Junctional adhesion molecule 3

Subcellular Location

Extracellular space, Cell membrane, Single-pass type I membrane protein, desmosome

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099 O.D. 0.088 0.134 0.176 0.265 0.402 0.702 1.263 2.099

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.