Mouse JAM-C/JAM3 ELISA Kit PicoKine®

JAM3 belongs to the junctional adhesion molecule protein family and functions as a receptor for another member of this family. This gene is mapped to 9; 9 A4. JAM3 plays a part in cell-cell adhesion. The soluble form of JAM3 is a mediator of angiogenesis. JAM3 is a counter-receptor for ITGAM, mediating leukocyteplatelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . A mutation in an intron of the JAM3 gene is linked with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 49 54 3.24 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 3 884 818 866 930 874 40.10 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 58 54 55 49 54 3.24 6.0% Sample 1 58 54 55 49 54 3.24 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 2 270 320 291 297 294 17.81 6.0% Sample 3 884 818 866 930 874 40.10 4.5% Sample 3 884 818 866 930 874 40.10 4.5%

Atherosclerosis, Cardiovascular, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Platelets, Signal Transduction, Tags & Cell Markers, Vascular Inflammation, Vasculature

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 58 270 884 61 279 991 Mean (pg/ml) 58 270 884 61 279 991 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 Standard deviation 2.90 14.04 39.78 2.81 17.86 74.33 CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5% CV (%) 5.0% 5.2% 4.5% 4.6% 6.4% 7.5%

Participates in cell-cell adhesion. It is a counter-receptor for ITGAM mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) . The soluble form is a mediator of angio

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016 O.D. 0.051 0.096 0.131 0.207 0.363 0.646 1.115 2.016

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.