Human Plexin B2/PLXNB2 ELISA Kit PicoKine®

Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 3 19603 18628 19571 18283 19021 578.86 3.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 1 1214 1018 1165 1017 1103 87.72 7.9% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 2 4275 4570 4511 4741 4524 166.86 3.6% Sample 3 19603 18628 19571 18283 19021 578.86 3.0% Sample 3 19603 18628 19571 18283 19021 578.86 3.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Mean (pg/ml) 1214 4275 19603 1233 4407 19741 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 Standard deviation 63.13 239.4 960.55 82.6 339.3 1539.8 CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8% CV (%) 5.2% 5.6% 4.9% 6.7% 7.7% 7.8%

Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239 O.D. 0.054 0.124 0.174 0.33 0.54 0.935 1.605 2.239

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.