Human LYPD3 ELISA Kit PicoKine®

Human LYPD3 ELISA Kit PicoKine® (96 Tests) . Quantitate Human LYPD3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 19q13.31.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 604 542 577 23.17 4.0% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 3 10613 9014 10567 8909 9775 815.25 8.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 590 572 604 542 577 23.17 4.0% Sample 1 590 572 604 542 577 23.17 4.0% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 2 2019 2207 2161 2108 2123 69.89 3.2% Sample 3 10613 9014 10567 8909 9775 815.25 8.3% Sample 3 10613 9014 10567 8909 9775 815.25 8.3%

Synonyms

Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4; LYPD3; C4.4A

Gene Name

LYPD3

Gene ID

27076

UniProt

O95274

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L31-C326

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TC

Applications

ELISA

Field of Research

Cancer, Tumor Biomarkers

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

0.312 ng/mL - 20 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 590 2019 10613 621 2312 11100 Mean (pg/ml) 590 2019 10613 621 2312 11100 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 Standard deviation 25.96 102.97 583.72 40.99 171.09 843.60 CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6% CV (%) 4.4% 5.1% 5.5% 6.6% 7.4% 7.6%

Sensitivity

<0.1 ng/mL

Function

Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human LYPD3

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

LY6/PLAUR domain containing 3

Subcellular Location

Cell membrane, GPI-anchor

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926 O.D. 0.031 0.075 0.117 0.189 0.355 0.549 0.995 1.926

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.