Human CES1 ELISA Kit PicoKine®

Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene is mapped to 16q12.2. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1486 1472 1378 1422 1439 42.74 5.3% Sample 2 5674 5344 5944 5180 5535 295.42 5.3% Sample 3 24323 25116 24596 24329 24591 322.53 1.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1486 1472 1378 1422 1439 42.74 5.3% Sample 1 1486 1472 1378 1422 1439 42.74 5.3% Sample 2 5674 5344 5944 5180 5535 295.42 5.3% Sample 2 5674 5344 5944 5180 5535 295.42 5.3% Sample 3 24323 25116 24596 24329 24591 322.53 1.3% Sample 3 24323 25116 24596 24329 24591 322.53 1.3%

Liver carboxylesterase 1; cholesterol acyltransferase; ACAT; Brain carboxylesterase hBr1; Carboxylesterase 1; CE-1; hCE-1; Cocaine carboxylesterase; Egasyn; HMSE; Methylumbelliferyl-acetate deacetylase 1; Monocyte/macrophage serine esterase; Retinyl ester

Cardiovascular, Cholesterol Metabolism, Drug Metabolism, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 CV (%) 4.2% 4.4% 5.3% 6.5% 5.1% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 CV (%) 4.2% 4.4% 5.3% 6.5% 5.1% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Mean (pg/ml) 1486 5674 24323 1499 6001 24678 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 Standard deviation 62.41 249.66 1289.12 97.44 306.05 1801.49 CV (%) 4.2% 4.4% 5.3% 6.5% 5.1% 7.3% CV (%) 4.2% 4.4% 5.3% 6.5% 5.1% 7.3%

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.1 0.164 0.327 0.557 0.959 1.776 2.49 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.1 0.164 0.327 0.557 0.959 1.776 2.49 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.044 0.1 0.164 0.327 0.557 0.959 1.776 2.49 O.D. 0.044 0.1 0.164 0.327 0.557 0.959 1.776 2.49

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.