Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®

Mouse Plexin C1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Plxnc1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 149 160 163 160 6.96 4.3% Sample 2 824 814 878 902 854 36.67 4.2% Sample 3 2614 2932 2991 2861 2849 143.54 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 149 160 163 160 6.96 4.3% Sample 1 168 149 160 163 160 6.96 4.3% Sample 2 824 814 878 902 854 36.67 4.2% Sample 2 824 814 878 902 854 36.67 4.2% Sample 3 2614 2932 2991 2861 2849 143.54 5.0% Sample 3 2614 2932 2991 2861 2849 143.54 5.0%

Synonyms

Plexin-C1; Virus-encoded semaphorin protein receptor; CD232; Plxnc1; Vespr

Gene Name

Plxnc1

Gene ID

54712

UniProt

Q9QZC2

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A35-T950

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Detected on dendritic cells, skin Langerhans cells and neutrophils.

Applications

ELISA

Field of Research

Cancer, Cell Adhesion, Invasion/Microenvironment

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

93.8 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6%

Sensitivity

<50 pg/mL

Function

Receptor for SEMA7A, for vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Plexin C1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Plexin C1

Subcellular Location

Membrane, Single-pass type I membrane protein

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.