Mouse Plexin C1/PLXNC1 ELISA Kit PicoKine®

A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 149 160 163 160 6.96 4.3% Sample 2 824 814 878 902 854 36.67 4.2% Sample 3 2614 2932 2991 2861 2849 143.54 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 149 160 163 160 6.96 4.3% Sample 1 168 149 160 163 160 6.96 4.3% Sample 2 824 814 878 902 854 36.67 4.2% Sample 2 824 814 878 902 854 36.67 4.2% Sample 3 2614 2932 2991 2861 2849 143.54 5.0% Sample 3 2614 2932 2991 2861 2849 143.54 5.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 168 824 2614 172 830 2761 Mean (pg/ml) 168 824 2614 172 830 2761 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 Standard deviation 7.2 47.8 162.07 11.35 48.97 209.8 CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6% CV (%) 4.3% 5.8% 6.2% 6.6% 5.9% 7.6%

Receptor for SEMA7A, for vaccinia virus semaphorin A39R and for herpesvirus Sema protein. Binding of semaphorins triggers cellular responses leading to the rearrangement of the cytoskeleton and to secretion of IL6 and IL8.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026 O.D. 0.035 0.136 0.244 0.399 0.66 1.129 1.624 2.026

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.