Mouse LYPD3 ELISA Kit PicoKine®

Mouse LYPD3 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Lypd3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 7; 7 A3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 110 102 99 105 5.12 4.8% Sample 2 913 945 924 872 913 26.57 2.9% Sample 3 2985 3342 3284 2927 3134 180.84 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 110 102 99 105 5.12 4.8% Sample 1 111 110 102 99 105 5.12 4.8% Sample 2 913 945 924 872 913 26.57 2.9% Sample 2 913 945 924 872 913 26.57 2.9% Sample 3 2985 3342 3284 2927 3134 180.84 5.7% Sample 3 2985 3342 3284 2927 3134 180.84 5.7%

Synonyms

Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Lypd3; C4.4a

Gene Name

Lypd3

Gene ID

72434

UniProt

Q91YK8

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L33-S343

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Up-regulated in suprabasal keratinocytes of hyperplastic skin induced by phorbol-ester.

Applications

ELISA

Field of Research

Cancer, Tumor Biomarkers

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

93.6 pg/mL - 6,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 913 2985 169 920 2991 Standard deviation 4.66 41.09 152.2 9.97 58.88 224.3 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 913 2985 169 920 2991 Standard deviation 4.66 41.09 152.2 9.97 58.88 224.3 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 913 2985 169 920 2991 Mean (pg/ml) 111 913 2985 169 920 2991 Standard deviation 4.66 41.09 152.2 9.97 58.88 224.3 Standard deviation 4.66 41.09 152.2 9.97 58.88 224.3 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5%

Sensitivity

<50 pg/mL

Function

Supports cell migration. May be involved in tumor progression.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse LYPD3

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Ly6/Plaur domain containing 3

Subcellular Location

Cell membrane, GPI-anchor

Sample Data

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.052 0.124 0.177 0.288 0.528 0.917 1.541 2.257 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.052 0.124 0.177 0.288 0.528 0.917 1.541 2.257 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.052 0.124 0.177 0.288 0.528 0.917 1.541 2.257 O.D. 0.052 0.124 0.177 0.288 0.528 0.917 1.541 2.257

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.