Rat Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine®

This gene is mapped to 19p11. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 226 230 236 230 230 3.57 1.5% Sample 2 1074 1096 1126 1007 1075 43.77 4.0% Sample 3 4954 4898 5293 4648 4948 229.97 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 226 230 236 230 230 3.57 1.5% Sample 1 226 230 236 230 230 3.57 1.5% Sample 2 1074 1096 1126 1007 1075 43.77 4.0% Sample 2 1074 1096 1126 1007 1075 43.77 4.0% Sample 3 4954 4898 5293 4648 4948 229.97 4.6% Sample 3 4954 4898 5293 4648 4948 229.97 4.6%

Carboxylesterase 1D; Carboxyesterase ES-10; Carboxylesterase 3; ES-HVEL; Fatty acid ethyl ester synthase; FAEE synthase; Liver carboxylesterase 10; pI 6.1 esterase; Ces1d; Ces3

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 226 1074 4954 239 1164 4979 Standard deviation 11.98 59.07 322.01 10.99 80.32 383.4 CV (%) 5.3% 5.5% 6.5% 4.6% 6.9% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 226 1074 4954 239 1164 4979 Standard deviation 11.98 59.07 322.01 10.99 80.32 383.4 CV (%) 5.3% 5.5% 6.5% 4.6% 6.9% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 226 1074 4954 239 1164 4979 Mean (pg/ml) 226 1074 4954 239 1164 4979 Standard deviation 11.98 59.07 322.01 10.99 80.32 383.4 Standard deviation 11.98 59.07 322.01 10.99 80.32 383.4 CV (%) 5.3% 5.5% 6.5% 4.6% 6.9% 7.7% CV (%) 5.3% 5.5% 6.5% 4.6% 6.9% 7.7%

Major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decre

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.091 0.163 0.299 0.552 1.016 1.573 2.081 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.091 0.163 0.299 0.552 1.016 1.573 2.081 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.091 0.163 0.299 0.552 1.016 1.573 2.081 O.D. 0.024 0.091 0.163 0.299 0.552 1.016 1.573 2.081

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.