Human CES2 ELISA Kit PicoKine®

Carboxylesterase 2 is an enzyme that in humans is encoded by the CES2 gene. This gene is mapped to 16q22.1. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. The protein encoded by this gene is the major intestinal enzyme and functions in intestine drug clearance. Alternatively spliced transcript variants have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 496 498 478 512 496 12.08 2.4% Sample 2 1686 1621 1692 1913 1728 110.37 6.3% Sample 3 8521 8662 9564 9565 9078 489.04 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 496 498 478 512 496 12.08 2.4% Sample 1 496 498 478 512 496 12.08 2.4% Sample 2 1686 1621 1692 1913 1728 110.37 6.3% Sample 2 1686 1621 1692 1913 1728 110.37 6.3% Sample 3 8521 8662 9564 9565 9078 489.04 5.3% Sample 3 8521 8662 9564 9565 9078 489.04 5.3%

Preferentially expressed in intestine with moderate expression in liver. Within the intestine, highest expression is found in small intestine with lower expression in colon and rectum.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 496 1686 8521 537 1912 9483 Standard deviation 20.83 82.6 434.57 30.1 122.37 673.3 CV (%) 4.2% 4.9% 5.1% 5.6% 6.4% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 496 1686 8521 537 1912 9483 Standard deviation 20.83 82.6 434.57 30.1 122.37 673.3 CV (%) 4.2% 4.9% 5.1% 5.6% 6.4% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 496 1686 8521 537 1912 9483 Mean (pg/ml) 496 1686 8521 537 1912 9483 Standard deviation 20.83 82.6 434.57 30.1 122.37 673.3 Standard deviation 20.83 82.6 434.57 30.1 122.37 673.3 CV (%) 4.2% 4.9% 5.1% 5.6% 6.4% 7.1% CV (%) 4.2% 4.9% 5.1% 5.6% 6.4% 7.1%

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Shows high catalytic efficiency for hydrolysis of cocaine, 4-methylumbelliferyl acetate, heroin and 6-monoacetylmorphine.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.103 0.15 0.246 0.501 0.816 1.277 1.89 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.103 0.15 0.246 0.501 0.816 1.277 1.89 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.029 0.103 0.15 0.246 0.501 0.816 1.277 1.89 O.D. 0.029 0.103 0.15 0.246 0.501 0.816 1.277 1.89

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.