Human CD164 ELISA Kit PicoKine®

Sialomucin core protein 24 also known as endolyn or CD164, is a protein that in humans is encoded by the CD164 gene. CD164 functions as a cell adhesion molecule. This gene is mapped to 6q21. The encoded protein also interacts with the C-X-C chemokine receptor type 4 and may regulate muscle development. Elevated expression of this gene has been observed in human patients with Sezary syndrome, a type of blood cancer, and a mutation in this gene may be associated with impaired hearing.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 135 144 154 145 7.01 4.8% Sample 2 717 672 786 731 726 40.68 5.6% Sample 3 2964 3060 3039 3034 3049 62.59 2.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 149 135 144 154 145 7.01 4.8% Sample 1 149 135 144 154 145 7.01 4.8% Sample 2 717 672 786 731 726 40.68 5.6% Sample 2 717 672 786 731 726 40.68 5.6% Sample 3 2964 3060 3039 3034 3049 62.59 2.0% Sample 3 2964 3060 3039 3034 3049 62.59 2.0%

Isoform 1 and isoform 3 are expressed in hematopoietic and non-hematopoietic tissues. Isoform 1 is expressed by prostate cancer tumors and prostate cancer cell lines. The expression is greater in bone metastases than in primary tumors. Expression in osseo

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 149 717 2964 162 741 2982 Mean (pg/ml) 149 717 2964 162 741 2982 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 Standard deviation 8.2 29.4 168.95 10.04 48.17 217.7 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3%

Sialomucin that may play a key role in hematopoiesis by facilitating the adhesion of CD34+ cells to the stroma and by negatively regulating CD34+CD38 (lo/-) cell proliferation. Modulates the migration of umbilical cord blood CD133+ cells and this is mediat

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149 O.D. 0.028 0.167 0.301 0.464 0.745 1.197 1.752 2.149

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.