Mouse TNFRSF12A/TWEAKR ELISA Kit PicoKine®

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 17; 17 A3.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 300 325 308 330 315 12.21 3.8% Sample 2 2167 2176 2072 2103 2129 43.52 2.0% Sample 3 4789 4265 4165 4933 4538 328.89 7.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 300 325 308 330 315 12.21 3.8% Sample 1 300 325 308 330 315 12.21 3.8% Sample 2 2167 2176 2072 2103 2129 43.52 2.0% Sample 2 2167 2176 2072 2103 2129 43.52 2.0% Sample 3 4789 4265 4165 4933 4538 328.89 7.2% Sample 3 4789 4265 4165 4933 4538 328.89 7.2%

Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Fibroblast growth factor-regulated protein 2; Tweak-receptor; TweakR; CD266; Tnfrsf12a; Fgfrp2; Fn14

Highly expressed in fetal heart, intestine, kidney, liver, lung and skin, and in adult heart and ovary. Intermediate expression in adult kidney, lung and skin.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 300 2167 4789 307 2379 4841 Standard deviation 18.3 143.02 258.6 13.2 114.19 363.08 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 300 2167 4789 307 2379 4841 Standard deviation 18.3 143.02 258.6 13.2 114.19 363.08 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 300 2167 4789 307 2379 4841 Mean (pg/ml) 300 2167 4789 307 2379 4841 Standard deviation 18.3 143.02 258.6 13.2 114.19 363.08 Standard deviation 18.3 143.02 258.6 13.2 114.19 363.08 CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5% CV (%) 6.1% 6.6% 5.4% 4.3% 4.8% 7.5%

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.11 0.177 0.334 0.587 0.994 1.635 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.11 0.177 0.334 0.587 0.994 1.635 2.389 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.11 0.177 0.334 0.587 0.994 1.635 2.389 O.D. 0.021 0.11 0.177 0.334 0.587 0.994 1.635 2.389

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.