Mouse Contactin-1 ELISA Kit PicoKine®

Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. This gene is mapped to 15 E3; 15 46.39 cM. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 298 288 323 291 300 13.76 4.5% Sample 2 1189 1277 1139 1303 1227 66.07 5.3% Sample 3 4684 4230 4964 4885 4690 284.92 6.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 298 288 323 291 300 13.76 4.5% Sample 1 298 288 323 291 300 13.76 4.5% Sample 2 1189 1277 1139 1303 1227 66.07 5.3% Sample 2 1189 1277 1139 1303 1227 66.07 5.3% Sample 3 4684 4230 4964 4885 4690 284.92 6.0% Sample 3 4684 4230 4964 4885 4690 284.92 6.0%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 298 1189 4684 303 1194 4854 Standard deviation 12.22 61.83 257.62 19.09 88.34 378.6 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 298 1189 4684 303 1194 4854 Standard deviation 12.22 61.83 257.62 19.09 88.34 378.6 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 298 1189 4684 303 1194 4854 Mean (pg/ml) 298 1189 4684 303 1194 4854 Standard deviation 12.22 61.83 257.62 19.09 88.34 378.6 Standard deviation 12.22 61.83 257.62 19.09 88.34 378.6 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8%

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association w

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.068 0.123 0.245 0.485 0.907 1.482 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.068 0.123 0.245 0.485 0.907 1.482 2.05 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.002 0.068 0.123 0.245 0.485 0.907 1.482 2.05 O.D. 0.002 0.068 0.123 0.245 0.485 0.907 1.482 2.05

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.