Human EphA1 ELISA Kit PicoKine®

EPHA1, EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The EPHA1 gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 221 221 214 211 12.73 6.0% Sample 2 1195 1276 1291 1165 1231 53.09 4.3% Sample 3 4507 4106 3971 4273 4214 200.02 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 190 221 221 214 211 12.73 6.0% Sample 1 190 221 221 214 211 12.73 6.0% Sample 2 1195 1276 1291 1165 1231 53.09 4.3% Sample 2 1195 1276 1291 1165 1231 53.09 4.3% Sample 3 4507 4106 3971 4273 4214 200.02 4.7% Sample 3 4507 4106 3971 4273 4214 200.02 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 1195 4507 244 1203 4632 Standard deviation 8.55 56.2 238.9 14.2 74.59 352.03 CV (%) 4.5% 4.7% 5.3% 5.8% 6.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 1195 4507 244 1203 4632 Standard deviation 8.55 56.2 238.9 14.2 74.59 352.03 CV (%) 4.5% 4.7% 5.3% 5.8% 6.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 1195 4507 244 1203 4632 Mean (pg/ml) 190 1195 4507 244 1203 4632 Standard deviation 8.55 56.2 238.9 14.2 74.59 352.03 Standard deviation 8.55 56.2 238.9 14.2 74.59 352.03 CV (%) 4.5% 4.7% 5.3% 5.8% 6.2% 7.6% CV (%) 4.5% 4.7% 5.3% 5.8% 6.2% 7.6%

Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent biional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.068 0.119 0.202 0.409 0.787 1.459 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.068 0.119 0.202 0.409 0.787 1.459 2.089 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.013 0.068 0.119 0.202 0.409 0.787 1.459 2.089 O.D. 0.013 0.068 0.119 0.202 0.409 0.787 1.459 2.089

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.