Human ROBO1 ELISA Kit PicoKine®

Human ROBO1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ROBO1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. This gene is mapped to 3p12.3. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 115 97 115 109 7.46 6.8% Sample 2 381 396 415 397 397 12.04 3.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 115 97 115 109 7.46 6.8% Sample 1 112 115 97 115 109 7.46 6.8% Sample 2 381 396 415 397 397 12.04 3.0% Sample 2 381 396 415 397 397 12.04 3.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0% Sample 3 1617 1731 1808 1764 1730 70.72 4.0%

Synonyms

Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1

Gene Name

ROBO1

Gene ID

6091

UniProt

Q9Y6N7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q26-P897

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Widely expressed, with exception of kidney.

Applications

ELISA

Field of Research

Angiogenesis, Axonal Guidance Proteins, Cancer, Growth and Development, Invasion/Microenvironment, Neurogenesis, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 381 1617 121 423 1768 Mean (pg/ml) 112 381 1617 121 423 1768 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 Standard deviation 5.71 20.4 69.53 5.93 28.3 139.7 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9%

Sensitivity

<10 pg/mL

Function

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ROBO1

Anticoagulant

Heparin

Gene Name Synonym

Roundabout guidance receptor 1

Subcellular Location

Cell membrane, Single-pass type I membrane protein, axon

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129 O.D. 0.007 0.08 0.153 0.274 0.49 0.896 1.512 2.129

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.