Human Contactin-1 ELISA Kit PicoKine®

Contactin 1, also known as CNTN1, is a protein which in humans is encoded by the CNTN1 gene. This gene is mapped to 12q12. The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI) -anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 346 337 354 335 19.01 5.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 3 4977 4553 5102 5081 4928 221.76 4.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 304 346 337 354 335 19.01 5.6% Sample 1 304 346 337 354 335 19.01 5.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 2 1040 1033 1054 1079 1051 17.58 1.6% Sample 3 4977 4553 5102 5081 4928 221.76 4.5% Sample 3 4977 4553 5102 5081 4928 221.76 4.5%

Strongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 304 1040 4977 310 1115 4983 Mean (pg/ml) 304 1040 4977 310 1115 4983 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 Standard deviation 19.15 59.28 238.9 20.46 81.4 383.7 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7%

Contactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association w

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898 O.D. 0.022 0.066 0.105 0.186 0.336 0.598 1.116 1.898

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.