Human TNFRSF12A/TWEAKR ELISA Kit PicoKine®

Human TNFRSF12A/TWEAKR ELISA Kit PicoKine® (96 Tests) . Quantitate Human TNFRSF12A in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 47 51 54 49 2.94 6.0% Sample 2 209 195 217 190 202 10.77 5.3% Sample 3 855 760 804 881 825 46.64 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 47 47 51 54 49 2.94 6.0% Sample 1 47 47 51 54 49 2.94 6.0% Sample 2 209 195 217 190 202 10.77 5.3% Sample 2 209 195 217 190 202 10.77 5.3% Sample 3 855 760 804 881 825 46.64 5.6% Sample 3 855 760 804 881 825 46.64 5.6%

Synonyms

Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD266; TNFRSF12A; FN14

Gene Name

TNFRSF12A

Gene ID

51330

UniProt

Q9NP84

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E28-P80

Capture Antibody

The capture antibody is polyclonal antibody from goat

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 47 209 855 58 247 903 Mean (pg/ml) 47 209 855 58 247 903 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 Standard deviation 2.21 11.5 50.45 3.71 16.55 67.8 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5%

Sensitivity

<10 pg/mL

Function

Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human TNFRSF12A/TWEAKR

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

TNF receptor superfamily member 12A

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963 O.D. 0.029 0.21 0.299 0.431 0.641 0.929 1.346 1.963

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.