Human Annexin A1 ELISA Kit PicoKine®

Human Annexin A1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ANXA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 100pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca (2+) -dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca (2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 299 282 293 299 293 6.94 2.3% Sample 2 1474 1425 1389 1444 1433 30.83 2.1% Sample 3 5209 4826 5016 4992 5010 135.84 2.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 299 282 293 299 293 6.94 2.3% Sample 1 299 282 293 299 293 6.94 2.3% Sample 2 1474 1425 1389 1444 1433 30.83 2.1% Sample 2 1474 1425 1389 1444 1433 30.83 2.1% Sample 3 5209 4826 5016 4992 5010 135.84 2.7% Sample 3 5209 4826 5016 4992 5010 135.84 2.7%

Synonyms

Annexin A1; Annexin I; Annexin-1; Calpactin II; Calpactin-2; Chromobindin-9; Lipocortin I; Phospholipase A2 inhibitory protein; p35; ANXA1; ANX1; LPC1

Gene Name

ANXA1

Gene ID

301

UniProt

P04083

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A2-N346

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Detected in resting neutrophils. Detected in peripheral blood T-cells. Detected in extracellular vesicles in blood serum from patients with inflammatory bowel disease, but not in serum from healthy donors. Detected in placenta (at protein level) . Detected

Applications

ELISA

Field of Research

Calcium Signaling, Cell Adhesion, Cytoskeleton/ECM, Lipid Signaling, Signal Transduction, Signaling Pathway

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 299 1474 5209 303 1499 5311 Standard deviation 12.56 64.86 276.1 17.9 92.9 414.3 CV (%) 4.2% 4.4% 5.3% 5.9% 6.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 299 1474 5209 303 1499 5311 Standard deviation 12.56 64.86 276.1 17.9 92.9 414.3 CV (%) 4.2% 4.4% 5.3% 5.9% 6.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 299 1474 5209 303 1499 5311 Mean (pg/ml) 299 1474 5209 303 1499 5311 Standard deviation 12.56 64.86 276.1 17.9 92.9 414.3 Standard deviation 12.56 64.86 276.1 17.9 92.9 414.3 CV (%) 4.2% 4.4% 5.3% 5.9% 6.2% 7.8% CV (%) 4.2% 4.4% 5.3% 5.9% 6.2% 7.8%

Sensitivity

<100 pg/mL

Function

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity. Plays a role in glucocorticoid-mediated down-regulation of the early phase of t

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Annexin A1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Annexin A1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.184 0.356 0.681 1.259 1.998 2.62 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.184 0.356 0.681 1.259 1.998 2.62 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.101 0.184 0.356 0.681 1.259 1.998 2.62 O.D. 0.025 0.101 0.184 0.356 0.681 1.259 1.998 2.62

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.