Rat BMP-10 ELISA Kit PicoKine®

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 4q34. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 33 32 34 2.27 6.6% Sample 1 34 38 33 32 34 2.27 6.6% Sample 2 244 232 243 247 241 5.67 2.3% Sample 2 244 232 243 247 241 5.67 2.3% Sample 3 404 404 433 399 410 13.43 3.2% Sample 3 404 404 433 399 410 13.43 3.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 244 404 37 249 456 Mean (pg/ml) 34 244 404 37 249 456 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 Standard deviation 1.87 12.4 17.38 2.26 16.4 34.2 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5%

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and N

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.086 0.132 0.241 0.432 0.753 1.395 2.207 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.086 0.132 0.241 0.432 0.753 1.395 2.207 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.02 0.086 0.132 0.241 0.432 0.753 1.395 2.207 O.D. 0.02 0.086 0.132 0.241 0.432 0.753 1.395 2.207

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.