Mouse BMP-10 ELISA Kit PicoKine®

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 6; 6 D1. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life.  BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation.  It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes.  BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration.  Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 32 29 29 1.29 4.4% Sample 2 211 200 186 207 201 9.51 4.7% Sample 3 444 465 430 505 461 28.29 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 29 29 32 29 29 1.29 4.4% Sample 1 29 29 32 29 29 1.29 4.4% Sample 2 211 200 186 207 201 9.51 4.7% Sample 2 211 200 186 207 201 9.51 4.7% Sample 3 444 465 430 505 461 28.29 6.1% Sample 3 444 465 430 505 461 28.29 6.1%

In the embryo, expressed exclusively in the ventricular trabecular myocardium of the developing heart from E9.0-E13.5. By E16.5-E18.5, only detectable in atria. Highly expressed in the adult heart where it is found in the right atrium but not in the left

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 211 444 31 226 487 Standard deviation 1.19 9.5 27.53 1.71 13.1 37.01 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 29 211 444 31 226 487 Standard deviation 1.19 9.5 27.53 1.71 13.1 37.01 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 29 211 444 31 226 487 Mean (pg/ml) 29 211 444 31 226 487 Standard deviation 1.19 9.5 27.53 1.71 13.1 37.01 Standard deviation 1.19 9.5 27.53 1.71 13.1 37.01 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6%

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and N

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.058 0.09 0.158 0.306 0.588 1.241 2.235 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.058 0.09 0.158 0.306 0.588 1.241 2.235 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.033 0.058 0.09 0.158 0.306 0.588 1.241 2.235 O.D. 0.033 0.058 0.09 0.158 0.306 0.588 1.241 2.235

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.