Human Glypican-5/GPC5 ELISA Kit PicoKine®

Human Glypican-5/GPC5 ELISA Kit PicoKine® (96 Tests) . Quantitate Human GPC5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glypican-5 is a protein that in humans is encoded by the GPC5 gene. This gene is mapped to 13q31.3. Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 72 63 72 66 5.35 8.1% Sample 2 226 232 264 250 243 15.00 6.1% Sample 3 938 925 1033 1044 985 53.83 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 60 72 63 72 66 5.35 8.1% Sample 1 60 72 63 72 66 5.35 8.1% Sample 2 226 232 264 250 243 15.00 6.1% Sample 2 226 232 264 250 243 15.00 6.1% Sample 3 938 925 1033 1044 985 53.83 5.4% Sample 3 938 925 1033 1044 985 53.83 5.4%

Synonyms

Glypican-5; Secreted glypican-5; GPC5

Gene Name

GPC5

Gene ID

2262

UniProt

P78333

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-T554

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

In adult, primarily expressed in the brain. Also detected in fetal brain, lung and liver.

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 226 938 62 243 967 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 60 226 938 62 243 967 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 60 226 938 62 243 967 Mean (pg/ml) 60 226 938 62 243 967 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 Standard deviation 2.58 11.07 50.7 4.15 16.77 72.5 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5%

Sensitivity

<10 pg/mL

Function

Cell surface proteoglycan that bears heparan sulfate.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human Glypican-5/GPC5

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Glypican 5

Subcellular Location

Cell membrane, GPI-anchor, Extracellular side, Secreted glypican-5, extracellular space

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.276 0.391 0.529 0.775 1.063 1.542 2.083 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.276 0.391 0.529 0.775 1.063 1.542 2.083 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.018 0.276 0.391 0.529 0.775 1.063 1.542 2.083 O.D. 0.018 0.276 0.391 0.529 0.775 1.063 1.542 2.083

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.