Rat CXADR ELISA Kit PicoKine®

Rat CXADR ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Cxadr in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CXADR (Coxsackie virus and adenovirus receptor) is a protein that in humans is encoded by the CXADR gene, also known as CAR, CVB3-binding protein, this gene is mapped to 11q11. Coxsackievirus B-adenovirus receptor. The CAR cDNA encodes a predicted 365-amino acid polypeptide that contains a single transmembrane domain and is a member of the immunoglobulin superfamily. By Northern blot analysis, they detected highest expression of 1.4-kb and 6-kb CXADR transcripts in pancreas, brain, heart, small intestine, testis, and prostate, lower expression in liver and lung, and no expression in kidney, placenta, peripheral blood leukocytes, thymus, and spleen. In comparison, mouse Cxadr showed highest expression in liver, and lower levels in kidney, heart, lung, and brain. The protein encoded by this gene is a type I membrane receptor for group B coxsackie viruses and subgroup C adenoviruses. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. CAR is strongly expressed in the developing central nervous system. It functions as a homophilic and also as a heterophilic cell adhesion molecule through its interactions with extracellular matrix glycoproteins , such as: fibronectin, agrin, laminin-1 and tenascin-R.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 54 60 60 58 2.48 4.2% Sample 2 245 204 214 217 220 15.21 6.9% Sample 3 808 924 922 839 873 50.94 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 59 54 60 60 58 2.48 4.2% Sample 1 59 54 60 60 58 2.48 4.2% Sample 2 245 204 214 217 220 15.21 6.9% Sample 2 245 204 214 217 220 15.21 6.9% Sample 3 808 924 922 839 873 50.94 5.8% Sample 3 808 924 922 839 873 50.94 5.8%

Synonyms

Coxsackievirus and adenovirus receptor homolog; CAR; rCAR; Cxadr; Car

Gene Name

Cxadr

Gene ID

89843

UniProt

Q9R066

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-G237

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expressed in heart, brain, spleen, lung, liver, muscle, kidney, testis, spleen and skeletal muscle.

Applications

ELISA

Field of Research

Cell Adhesion, Cytoskeleton/ECM, Host-Virus Interaction, Interspecies Interaction, Microbiology, Signal Transduction, Tight Junctions

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 245 808 61 249 932 Standard deviation 3.6 11.52 45.25 3.9 18.2 72.7 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 59 245 808 61 249 932 Standard deviation 3.6 11.52 45.25 3.9 18.2 72.7 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 59 245 808 61 249 932 Mean (pg/ml) 59 245 808 61 249 932 Standard deviation 3.6 11.52 45.25 3.9 18.2 72.7 Standard deviation 3.6 11.52 45.25 3.9 18.2 72.7 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8%

Sensitivity

<10 pg/mL

Function

Component of the epithelial apical junction complex that may function as a homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with JAML a

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat CXADR

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

CXADR, Ig-like cell adhesion molecule

Subcellular Location

Cell membrane, Single-pass type I membrane protein, Basolateral cell membrane, tight junction, adherens junction

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.106 0.19 0.353 0.595 1.004 1.544 2.104 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.106 0.19 0.353 0.595 1.004 1.544 2.104 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.024 0.106 0.19 0.353 0.595 1.004 1.544 2.104 O.D. 0.024 0.106 0.19 0.353 0.595 1.004 1.544 2.104

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.