Human PROS1 / Protein S ELISA Kit PicoKine®

This PROS1 gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. This gene is mapped to 3q11.1. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4bbinding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 497 513 464 513 496 20.00 4.0% Sample 2 2158 2442 2327 2374 2325 104.86 4.5% Sample 3 8049 8437 8377 9288 8537 457.64 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 497 513 464 513 496 20.00 4.0% Sample 1 497 513 464 513 496 20.00 4.0% Sample 2 2158 2442 2327 2374 2325 104.86 4.5% Sample 2 2158 2442 2327 2374 2325 104.86 4.5% Sample 3 8049 8437 8377 9288 8537 457.64 5.3% Sample 3 8049 8437 8377 9288 8537 457.64 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 497 2158 8049 521 2354 9179 Standard deviation 20.87 92.79 330.01 34.39 160.07 651.71 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 497 2158 8049 521 2354 9179 Standard deviation 20.87 92.79 330.01 34.39 160.07 651.71 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 497 2158 8049 521 2354 9179 Mean (pg/ml) 497 2158 8049 521 2354 9179 Standard deviation 20.87 92.79 330.01 34.39 160.07 651.71 Standard deviation 20.87 92.79 330.01 34.39 160.07 651.71 CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1% CV (%) 4.2% 4.3% 4.1% 6.6% 6.8% 7.1%

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.097 0.17 0.3 0.515 0.928 1.591 2.279 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.097 0.17 0.3 0.515 0.928 1.591 2.279 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.097 0.17 0.3 0.515 0.928 1.591 2.279 O.D. 0.022 0.097 0.17 0.3 0.515 0.928 1.591 2.279

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.