Human Ki67 ELISA Kit PicoKine®

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. This gene is mapped to 10q26.2. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 17 17 15 16 1.00 6.2% Sample 2 54 64 64 54 59 5.00 8.4% Sample 3 222 213 228 245 227 11.68 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 15 17 17 15 16 1.00 6.2% Sample 1 15 17 17 15 16 1.00 6.2% Sample 2 54 64 64 54 59 5.00 8.4% Sample 2 54 64 64 54 59 5.00 8.4% Sample 3 222 213 228 245 227 11.68 5.1% Sample 3 222 213 228 245 227 11.68 5.1%

Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) . Present at highest level in G2 phase and during mitosis (at protein level) . In interphase

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 15 54 222 16 60 234 Mean (pg/ml) 15 54 222 16 60 234 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 Standard deviation 0.9 3.13 10.4 0.94 4.2 18.25 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8%

Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome su

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2 O.D. 0.039 0.108 0.186 0.321 0.599 1.017 1.586 2.2

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.