Human AMICA/JAML ELISA Kit PicoKine®

Human AMICA/JAML ELISA Kit PicoKine® (96 Tests) . Quantitate Human JAML in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member. This gene is mapped to 11q23.3. It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in humans and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells. JAML-mediated activation of CAR is required for neutrophil extravasation in addition to other leukocyte/epithelial cell interaction models. Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 110 107 108 1.78 1.6% Sample 2 451 458 536 485 482 33.39 6.9% Sample 3 1179 1111 1036 1140 1116 52.36 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 110 107 108 1.78 1.6% Sample 1 106 110 110 107 108 1.78 1.6% Sample 2 451 458 536 485 482 33.39 6.9% Sample 2 451 458 536 485 482 33.39 6.9% Sample 3 1179 1111 1036 1140 1116 52.36 4.6% Sample 3 1179 1111 1036 1140 1116 52.36 4.6%

Synonyms

Junctional adhesion molecule-like; Adhesion molecule interacting with CXADR antigen 1; Dendritic cell-specific protein CREA7-1; JAML; AMICA1; UNQ722; PRO1387

Gene Name

JAML

Gene ID

120425

UniProt

Q86YT9

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-L275

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes.

Applications

ELISA

Field of Research

Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton/ECM, Immunology, Signal Transduction, Tight Junctions

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 451 1179 110 500 1230 Standard deviation 4.45 20.8 62.5 7.04 34 88.56 CV (%) 4.2% 4.6% 5.3% 6.4% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 451 1179 110 500 1230 Standard deviation 4.45 20.8 62.5 7.04 34 88.56 CV (%) 4.2% 4.6% 5.3% 6.4% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 106 451 1179 110 500 1230 Mean (pg/ml) 106 451 1179 110 500 1230 Standard deviation 4.45 20.8 62.5 7.04 34 88.56 Standard deviation 4.45 20.8 62.5 7.04 34 88.56 CV (%) 4.2% 4.6% 5.3% 6.4% 6.8% 7.2% CV (%) 4.2% 4.6% 5.3% 6.4% 6.8% 7.2%

Sensitivity

<10 pg/mL

Function

Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors me

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human AMICA/JAML

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Junction adhesion molecule like

Subcellular Location

Cell membrane, Single-pass type I membrane protein, Cell junction

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.082 0.173 0.294 0.522 0.976 1.585 2.118 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.082 0.173 0.294 0.522 0.976 1.585 2.118 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.0 0.082 0.173 0.294 0.522 0.976 1.585 2.118 O.D. 0.0 0.082 0.173 0.294 0.522 0.976 1.585 2.118

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.