Human VWF-A2/VWF ELISA Kit PicoKine®

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of plateletvessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 571 552 614 586 580 22.66 3.9% Sample 2 1728 1659 1612 1716 1678 46.52 2.7% Sample 3 9762 9879 8855 8135 9157 711.16 7.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 571 552 614 586 580 22.66 3.9% Sample 1 571 552 614 586 580 22.66 3.9% Sample 2 1728 1659 1612 1716 1678 46.52 2.7% Sample 2 1728 1659 1612 1716 1678 46.52 2.7% Sample 3 9762 9879 8855 8135 9157 711.16 7.7% Sample 3 9762 9879 8855 8135 9157 711.16 7.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 571 1728 9762 600 1987 9834 Standard deviation 35.4 73.3 497.86 40.2 147.04 717.9 CV (%) 6.2% 4.3% 5.1% 6.7% 7.4% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 571 1728 9762 600 1987 9834 Standard deviation 35.4 73.3 497.86 40.2 147.04 717.9 CV (%) 6.2% 4.3% 5.1% 6.7% 7.4% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 571 1728 9762 600 1987 9834 Mean (pg/ml) 571 1728 9762 600 1987 9834 Standard deviation 35.4 73.3 497.86 40.2 147.04 717.9 Standard deviation 35.4 73.3 497.86 40.2 147.04 717.9 CV (%) 6.2% 4.3% 5.1% 6.7% 7.4% 7.3% CV (%) 6.2% 4.3% 5.1% 6.7% 7.4% 7.3%

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone f

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.151 0.243 0.47 0.82 1.267 1.765 2.075 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.151 0.243 0.47 0.82 1.267 1.765 2.075 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.022 0.151 0.243 0.47 0.82 1.267 1.765 2.075 O.D. 0.022 0.151 0.243 0.47 0.82 1.267 1.765 2.075

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.