Human ckmm ELISA Kit PicoKine®

Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.

Lots Lot 1 (mU/ml) Lot 2 (mU/ml) Lot 3 (mU/ml) Lot 4 (mU/ml) Mean (mU/ml) Standard Deviation CV (%) Sample 1 233 233 252 234 238 8.09 3.3% Sample 2 1369 1559 1602 1564 1523 90.73 5.9% Sample 3 3636 3701 3777 4293 3851 259.59 6.7% Lots Lot 1 (mU/ml) Lot 2 (mU/ml) Lot 3 (mU/ml) Lot 4 (mU/ml) Mean (mU/ml) Standard Deviation CV (%) Lots Lot 1 (mU/ml) Lot 2 (mU/ml) Lot 3 (mU/ml) Lot 4 (mU/ml) Mean (mU/ml) Standard Deviation CV (%) Sample 1 233 233 252 234 238 8.09 3.3% Sample 1 233 233 252 234 238 8.09 3.3% Sample 2 1369 1559 1602 1564 1523 90.73 5.9% Sample 2 1369 1559 1602 1564 1523 90.73 5.9% Sample 3 3636 3701 3777 4293 3851 259.59 6.7% Sample 3 3636 3701 3777 4293 3851 259.59 6.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (mU/ml) 233 1369 3636 242 1421 4520 Standard deviation 9.77 88.99 147.5 13.31 96.6 329.96 CV (%) 4.2% 6.5% 4.8% 5.5% 6.8% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (mU/ml) 233 1369 3636 242 1421 4520 Standard deviation 9.77 88.99 147.5 13.31 96.6 329.96 CV (%) 4.2% 6.5% 4.8% 5.5% 6.8% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (mU/ml) 233 1369 3636 242 1421 4520 Mean (mU/ml) 233 1369 3636 242 1421 4520 Standard deviation 9.77 88.99 147.5 13.31 96.6 329.96 Standard deviation 9.77 88.99 147.5 13.31 96.6 329.96 CV (%) 4.2% 6.5% 4.8% 5.5% 6.8% 7.3% CV (%) 4.2% 6.5% 4.8% 5.5% 6.8% 7.3%

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) . Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (mU/ml) 0 0.15 0.31 0.62 1.25 2.5 5 10 O.D. 0.027 0.095 0.142 0.247 0.465 0.746 1.316 1.861 Concentration (mU/ml) 0 0.15 0.31 0.62 1.25 2.5 5 10 O.D. 0.027 0.095 0.142 0.247 0.465 0.746 1.316 1.861 Concentration (mU/ml) 0 0.15 0.31 0.62 1.25 2.5 5 10 Concentration (mU/ml) 0 0.15 0.31 0.62 1.25 2.5 5 10 O.D. 0.027 0.095 0.142 0.247 0.465 0.746 1.316 1.861 O.D. 0.027 0.095 0.142 0.247 0.465 0.746 1.316 1.861

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.