Rat IL6ST ELISA Kit PicoKine®

GP130 (Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1254 1275 1273 1502 1326 101.94 7.6% Sample 2 4597 4887 4558 4251 4573 225.32 4.9% Sample 3 20188 20369 19931 19988 20119 173.03 0.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1254 1275 1273 1502 1326 101.94 7.6% Sample 1 1254 1275 1273 1502 1326 101.94 7.6% Sample 2 4597 4887 4558 4251 4573 225.32 4.9% Sample 2 4597 4887 4558 4251 4573 225.32 4.9% Sample 3 20188 20369 19931 19988 20119 173.03 0.8% Sample 3 20188 20369 19931 19988 20119 173.03 0.8%

Interleukin-6 receptor subunit beta; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6R-beta; IL-6RB; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; Oncostatin-M receptor subunit alpha; CD130; Il6st

Adapters, Apoptosis, Associated Proteins, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Type Markers, Cytokines, Hypertrophy, Immunology, Intracellular, Signal Transduction, Transmembrane

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1254 4579 20188 1322 5546 21456 Standard deviation 66.46 290.3 847.9 99.15 321.7 1652.1 CV (%) 5.3% 6.1% 4.2% 7.5% 5.8% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1254 4579 20188 1322 5546 21456 Standard deviation 66.46 290.3 847.9 99.15 321.7 1652.1 CV (%) 5.3% 6.1% 4.2% 7.5% 5.8% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1254 4579 20188 1322 5546 21456 Mean (pg/ml) 1254 4579 20188 1322 5546 21456 Standard deviation 66.46 290.3 847.9 99.15 321.7 1652.1 Standard deviation 66.46 290.3 847.9 99.15 321.7 1652.1 CV (%) 5.3% 6.1% 4.2% 7.5% 5.8% 7.7% CV (%) 5.3% 6.1% 4.2% 7.5% 5.8% 7.7%

Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize IL6ST for initiating signal transmission. Binding of IL6 to IL6R induces IL6ST homodimerization and formation of a high-affinity receptor complex, w

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.124 0.178 0.289 0.605 1.026 1.63 2.209 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.124 0.178 0.289 0.605 1.026 1.63 2.209 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.054 0.124 0.178 0.289 0.605 1.026 1.63 2.209 O.D. 0.054 0.124 0.178 0.289 0.605 1.026 1.63 2.209

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.