Human CD14 ELISA Kit PicoKine®

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS) . CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP) . Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 311 305 362 322 23.20 7.2% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 3 4116 3778 3962 4374 4057 218.42 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 310 311 305 362 322 23.20 7.2% Sample 1 310 311 305 362 322 23.20 7.2% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 2 1057 1075 989 1027 1037 32.58 3.1% Sample 3 4116 3778 3962 4374 4057 218.42 5.3% Sample 3 4116 3778 3962 4374 4057 218.42 5.3%

Detected on macrophages (at protein level) . Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 310 1057 4116 312 1164 4732 Mean (pg/ml) 310 1057 4116 312 1164 4732 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 Standard deviation 13.95 49.7 209.92 18.1 75.66 350.2 CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4% CV (%) 4.5% 4.7% 5.1% 5.8% 6.5% 7.4%

Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) . Acts via MyD88, TIRAP and

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024 O.D. 0.033 0.095 0.155 0.259 0.42 0.759 1.264 2.024

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.