Human ITIH4 ELISA Kit PicoKine®

Human ITIH4 ELISA Kit PicoKine® (96 Tests) . Quantitate Human ITIH4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Inter-alpha-trypsin inhibitor heavy chain H4 is a protein that in humans is encoded by the ITIH4 gene. This gene is mapped to 3p21.1. The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 49 52 45 48 2.50 5.2% Sample 2 216 216 209 205 211 4.71 2.2% Sample 3 746 856 880 835 829 50.63 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 48 49 52 45 48 2.50 5.2% Sample 1 48 49 52 45 48 2.50 5.2% Sample 2 216 216 209 205 211 4.71 2.2% Sample 2 216 216 209 205 211 4.71 2.2% Sample 3 746 856 880 835 829 50.63 6.1% Sample 3 746 856 880 835 829 50.63 6.1%

Synonyms

Inter-alpha-trypsin inhibitor heavy chain H4; ITI heavy chain H4; ITI-HC4; Inter-alpha-inhibitor heavy chain 4; Inter-alpha-trypsin inhibitor family heavy chain-related protein; IHRP; Plasma kallikrein sensitive glycoprotein 120; Gp120; PK-120; ITIH4; IHR

Gene Name

ITIH4

Gene ID

3700

UniProt

Q14624

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E29-L930

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated detection polyclonal antibody from goat

Tissue Specificity

Liver specific.

Applications

ELISA

Field of Research

Cardiovascular, Immunology, Innate Immunity, Macrophage/Inflammation, Serum Proteins

Standard

2 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 216 746 58 243 852 Standard deviation 1.97 9.72 42.5 3.6 17.7 64.75 CV (%) 4.1% 4.5% 5.7% 6.2% 7.3% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 48 216 746 58 243 852 Standard deviation 1.97 9.72 42.5 3.6 17.7 64.75 CV (%) 4.1% 4.5% 5.7% 6.2% 7.3% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 48 216 746 58 243 852 Mean (pg/ml) 48 216 746 58 243 852 Standard deviation 1.97 9.72 42.5 3.6 17.7 64.75 Standard deviation 1.97 9.72 42.5 3.6 17.7 64.75 CV (%) 4.1% 4.5% 5.7% 6.2% 7.3% 7.6% CV (%) 4.1% 4.5% 5.7% 6.2% 7.3% 7.6%

Sensitivity

<10 pg/mL

Function

Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human ITIH4

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Inter-alpha-trypsin inhibitor heavy chain family member 4

Subcellular Location

Secreted

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.074 0.268 0.365 0.536 0.715 1.119 1.671 2.515 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.074 0.268 0.365 0.536 0.715 1.119 1.671 2.515 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.074 0.268 0.365 0.536 0.715 1.119 1.671 2.515 O.D. 0.074 0.268 0.365 0.536 0.715 1.119 1.671 2.515

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.