Human GSTA ELISA Kit PicoKine®

Human GSTA ELISA Kit PicoKine® (96 Tests) . Quantitate Human GSTA1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

GSTA encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 298 279 301 294 293 8.45 2.8% Sample . 2 1176 1300 1194 1293 1240 56.16 4.5%% Sample 3 6136 5932 6351 5515 5983 308.40 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 298 279 301 294 293 8.45 2.8% Sample 1 298 279 301 294 293 8.45 2.8% Sample . 2 1176 1300 1194 1293 1240 56.16 4.5%% Sample . 2 1176 1300 1194 1293 1240 56.16 4.5%% Sample 3 6136 5932 6351 5515 5983 308.40 5.1% Sample 3 6136 5932 6351 5515 5983 308.40 5.1%

Synonyms

Glutathione S-transferase A2; GST HA subunit 2; GST class-alpha member 2; GST-gamma; GSTA2-2; GTH2; GSTA2; GST2

Gene Name

GSTA1

Gene ID

2938

UniProt

P08263

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F222

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Liver.

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 298 1176 6136 305 1197 6246 Standard deviation 13.1 55.27 331.3 19.22 80.2 462.2 CV (%) 4.4% 4.7% 5.4% 6.3% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 298 1176 6136 305 1197 6246 Standard deviation 13.1 55.27 331.3 19.22 80.2 462.2 CV (%) 4.4% 4.7% 5.4% 6.3% 6.7% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 298 1176 6136 305 1197 6246 Mean (pg/ml) 298 1176 6136 305 1197 6246 Standard deviation 13.1 55.27 331.3 19.22 80.2 462.2 Standard deviation 13.1 55.27 331.3 19.22 80.2 462.2 CV (%) 4.4% 4.7% 5.4% 6.3% 6.7% 7.4% CV (%) 4.4% 4.7% 5.4% 6.3% 6.7% 7.4%

Sensitivity

<10 pg/mL

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human GSTA

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Glutathione S-transferase alpha 1

Subcellular Location

Cytoplasm.

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.