Rat FGF21 ELISA Kit PicoKine®

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 48 42 44 46 3.16 6.8% Sample 2 155 181 179 185 175 11.74 6.7% Sample 3 638 762 636 688 681 51.19 7.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 50 48 42 44 46 3.16 6.8% Sample 1 50 48 42 44 46 3.16 6.8% Sample 2 155 181 179 185 175 11.74 6.7% Sample 2 155 181 179 185 175 11.74 6.7% Sample 3 638 762 636 688 681 51.19 7.5% Sample 3 638 762 636 688 681 51.19 7.5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 155 638 57 172 749 Standard deviation 2.10 6.67 30.62 2.79 11.01 58.42 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 50 155 638 57 172 749 Standard deviation 2.10 6.67 30.62 2.79 11.01 58.42 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 50 155 638 57 172 749 Mean (pg/ml) 50 155 638 57 172 749 Standard deviation 2.10 6.67 30.62 2.79 11.01 58.42 Standard deviation 2.10 6.67 30.62 2.79 11.01 58.42 CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 7.8% CV (%) 4.2% 4.3% 4.8% 4.9% 6.4% 7.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.008 0.062 0.113 0.2 0.361 0.734 1.365 2.317 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.008 0.062 0.113 0.2 0.361 0.734 1.365 2.317 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.008 0.062 0.113 0.2 0.361 0.734 1.365 2.317 O.D. 0.008 0.062 0.113 0.2 0.361 0.734 1.365 2.317

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.