Rat P-Cadherin-3 CDH3 ELISA Kit PicoKine®

Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 32 33 30 2.06 6.8% Sample . 2 221 206 218 202 211 7.94 3.7%% Sample 3 384 382 382 417 387 18.82 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 28 29 32 33 30 2.06 6.8% Sample 1 28 29 32 33 30 2.06 6.8% Sample . 2 221 206 218 202 211 7.94 3.7%% Sample . 2 221 206 218 202 211 7.94 3.7%% Sample 3 384 382 382 417 387 18.82 4.8% Sample 3 384 382 382 417 387 18.82 4.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 221 384 30 229 407 Standard deviation 1.18 9.95 19.58 1.77 14.66 30.5 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 28 221 384 30 229 407 Standard deviation 1.18 9.95 19.58 1.77 14.66 30.5 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 28 221 384 30 229 407 Mean (pg/ml) 28 221 384 30 229 407 Standard deviation 1.18 9.95 19.58 1.77 14.66 30.5 Standard deviation 1.18 9.95 19.58 1.77 14.66 30.5 CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5% CV (%) 4.2% 4.5% 5.1% 5.9% 6.4% 7.5%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.05 0.089 0.167 0.333 0.602 1.296 2.357 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.05 0.089 0.167 0.333 0.602 1.296 2.357 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.008 0.05 0.089 0.167 0.333 0.602 1.296 2.357 O.D. 0.008 0.05 0.089 0.167 0.333 0.602 1.296 2.357

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.