Rat FGF23 ELISA Kit PicoKine®

Fibroblast growth factor 23 or FGF23 is a protein that in humans is encoded by the FGF23 gene. It is a member of the fibroblast growth factor (FGF) family which is responsible for phosphate metabolism. The main function of FGF23 seems to be regulation of phosphate concentration in plasma. FGF23 is secreted by Osteocytes in response to elevated Calcitriol. And it acts on the kidneys, where it decreases the expression of NPT2, a sodium-phosphate cotransporter in the proximal tubule. Thus, FGF23 decreases the reabsorption and increases excretion of phosphate. Also, FGF23 may suppress 1-alpha-hydroxylase, reducing its ability to activate vitamin D and subsequently impairing calcium absorption.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 53 49 53 3.26 6.1% Sample . 2 244 245 238 254 245 5.71 2.3% Sample 3 925 917 908 863 903 24.00 2.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 53 49 53 3.26 6.1% Sample 1 55 58 53 49 53 3.26 6.1% Sample . 2 244 245 238 254 245 5.71 2.3% Sample . 2 244 245 238 254 245 5.71 2.3% Sample 3 925 917 908 863 903 24.00 2.6% Sample 3 925 917 908 863 903 24.00 2.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 244 925 59 248 978 Standard deviation 2.81 13.18 39.88 2.9 16.6 77.3 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 244 925 59 248 978 Standard deviation 2.81 13.18 39.88 2.9 16.6 77.3 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 244 925 59 248 978 Mean (pg/ml) 55 244 925 59 248 978 Standard deviation 2.81 13.18 39.88 2.9 16.6 77.3 Standard deviation 2.81 13.18 39.88 2.9 16.6 77.3 CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9% CV (%) 5.1% 5.4% 4.3% 4.9% 6.7% 7.9%

Regulator of phosphate homeostasis (By similarity) . Inhibits renal tubular phosphate transport by reducing SLC34A1 levels (By similarity) . Regulator of vitamin-D metabolism (By similarity) . Negatively regulates osteoblasts differentiation and matrix mineralization (By similarity) . Acts ly on the parathyroid to decrease PTH secretion. Upregulates EGR1 expression in the presence of KL.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.123 0.187 0.303 0.531 0.922 1.428 1.983 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.123 0.187 0.303 0.531 0.922 1.428 1.983 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.123 0.187 0.303 0.531 0.922 1.428 1.983 O.D. 0.062 0.123 0.187 0.303 0.531 0.922 1.428 1.983

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.