Mouse EFEMP1 ELISA Kit PicoKine®

Mouse EFEMP1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Efemp1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

EGF-containing fibulin-like extracellular matrix protein 1 is a protein that in humans is encoded by the EFEMP1 gene. This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 685 744 804 735 742 40.81 5.5% Sample 2 1294 1365 1437 1545 1410 88.85 6.3% Sample 3 6512 7433 6841 8471 7314 416.91 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 685 744 804 735 742 40.81 5.5% Sample 1 685 744 804 735 742 40.81 5.5% Sample 2 1294 1365 1437 1545 1410 88.85 6.3% Sample 2 1294 1365 1437 1545 1410 88.85 6.3% Sample 3 6512 7433 6841 8471 7314 416.91 5.7% Sample 3 6512 7433 6841 8471 7314 416.91 5.7%

Synonyms

EGF-containing fibulin-like extracellular matrix protein 1; Fibulin-3; FIBL-3; Efemp1; Fbln3

Gene Name

Efemp1

Gene ID

216616

UniProt

Q8BPB5

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q18-F493

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Calcium Binding Proteins, Calcium Signaling, Neuroscience, Sensory System, Signal Transduction, Signaling Pathway, Visual System

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 685 1294 6512 705 1369 7056 Standard deviation 32.2 71.17 384.21 45.12 91.72 529.2 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 685 1294 6512 705 1369 7056 Standard deviation 32.2 71.17 384.21 45.12 91.72 529.2 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 685 1294 6512 705 1369 7056 Mean (pg/ml) 685 1294 6512 705 1369 7056 Standard deviation 32.2 71.17 384.21 45.12 91.72 529.2 Standard deviation 32.2 71.17 384.21 45.12 91.72 529.2 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5%

Sensitivity

<10 pg/mL

Function

Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth (By similarity) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse EFEMP1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Epidermal growth factor-containing fibulin-like extracellular matrix protein 1

Subcellular Location

Secreted, extracellular space . Secreted, extracellular space, extracellular matrix . Localizes to the lamina propria underneath the olfactory epithelium.

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.121 0.175 0.314 0.519 0.99 1.775 2.383 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.121 0.175 0.314 0.519 0.99 1.775 2.383 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.048 0.121 0.175 0.314 0.519 0.99 1.775 2.383 O.D. 0.048 0.121 0.175 0.314 0.519 0.99 1.775 2.383

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.