Rat Galectin-1 ELISA Kit PicoKine®

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 243 248 224 249 241 10.07 4.1% Sample . 2 1504 1287 1331 1330 1363 83.32 6.1%% Sample 3 5437 6005 5471 5982 5723 270.14 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 243 248 224 249 241 10.07 4.1% Sample 1 243 248 224 249 241 10.07 4.1% Sample . 2 1504 1287 1331 1330 1363 83.32 6.1%% Sample . 2 1504 1287 1331 1330 1363 83.32 6.1%% Sample 3 5437 6005 5471 5982 5723 270.14 4.7% Sample 3 5437 6005 5471 5982 5723 270.14 4.7%

Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; RL 14.5; S-Lac lectin 1; Lgals1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7%

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.071 0.15 0.301 0.575 1.078 1.679 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.071 0.15 0.301 0.575 1.078 1.679 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.005 0.071 0.15 0.301 0.575 1.078 1.679 2.03 O.D. 0.005 0.071 0.15 0.301 0.575 1.078 1.679 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.