Human HRG ELISA Kit PicoKine®

Human HRG ELISA Kit PicoKine® (96 Tests) . Quantitate Human HRG in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 264 246 248 250 7.92 3.1% Sample . 2 1957 2089 1985 1978 2002 51.13 2.5%% Sample 3 4517 4462 4358 4566 4475 77.29 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 244 264 246 248 250 7.92 3.1% Sample 1 244 264 246 248 250 7.92 3.1% Sample . 2 1957 2089 1985 1978 2002 51.13 2.5%% Sample . 2 1957 2089 1985 1978 2002 51.13 2.5%% Sample 3 4517 4462 4358 4566 4475 77.29 1.7% Sample 3 4517 4462 4358 4566 4475 77.29 1.7%

Synonyms

Heme transporter HRG1; Heme-responsive gene 1 protein homolog; HRG-1; hHRG-1; Solute carrier family 48 member 1; SLC48A1; HRG1

Gene Name

HRG

Gene ID

3273

UniProt

Q6P1K1

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: V19-K525

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Highly expressed in the brain, kidney, heart and skeletal muscle. Moderately expressed in the liver, lung, placenta and small intestine.

Field of Research

Cancer, Neural Signal Transduction, Neurology Process, Neuroscience

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 1957 4517 246 1967 4596 Standard deviation 10.49 95.89 243.9 16.48 135.7 344.7 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 1957 4517 246 1967 4596 Standard deviation 10.49 95.89 243.9 16.48 135.7 344.7 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 244 1957 4517 246 1967 4596 Mean (pg/ml) 244 1957 4517 246 1967 4596 Standard deviation 10.49 95.89 243.9 16.48 135.7 344.7 Standard deviation 10.49 95.89 243.9 16.48 135.7 344.7 CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5% CV (%) 4.3% 4.9% 5.4% 6.7% 6.9% 7.5%

Sensitivity

<10 pg/mL

Function

Heme transporter that regulates intracellular heme availability through the endosomal or lysosomal compartment.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human HRG

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Histidine rich glycoprotein

Subcellular Location

Endosome membrane

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.045 0.085 0.158 0.328 0.591 1.152 1.943 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.045 0.085 0.158 0.328 0.591 1.152 1.943 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.003 0.045 0.085 0.158 0.328 0.591 1.152 1.943 O.D. 0.003 0.045 0.085 0.158 0.328 0.591 1.152 1.943

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.