Human Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1883 2175 2014 2130 2050 113.14 5.5% Sample . 2 10402 11339 11464 10592 10949 459.34 4.1%% Sample 3 40754 39524 41038 45998 41828 2473.60 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1883 2175 2014 2130 2050 113.14 5.5% Sample 1 1883 2175 2014 2130 2050 113.14 5.5% Sample . 2 10402 11339 11464 10592 10949 459.34 4.1%% Sample . 2 10402 11339 11464 10592 10949 459.34 4.1%% Sample 3 40754 39524 41038 45998 41828 2473.60 5.9% Sample 3 40754 39524 41038 45998 41828 2473.60 5.9%

Angiogenesis, Cardiovascular, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Visual System

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1883 10402 40754 1930 12110 43978 Standard deviation 114.86 488.89 2282.2 123.52 884.03 3430.28 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1883 10402 40754 1930 12110 43978 Standard deviation 114.86 488.89 2282.2 123.52 884.03 3430.28 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1883 10402 40754 1930 12110 43978 Mean (pg/ml) 1883 10402 40754 1930 12110 43978 Standard deviation 114.86 488.89 2282.2 123.52 884.03 3430.28 Standard deviation 114.86 488.89 2282.2 123.52 884.03 3430.28 CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8% CV (%) 6.1% 4.7% 5.6% 6.4% 7.3% 7.8%

Acts as a carrier protein that release fibroblast- binding factors (FGFs) from the extracellular matrix (EM) storage and thus enhance the mitogenic activity of FGFs. Enhances FGF2 signaling during tissue repair, angiogenesis and in tumor growth (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.007 0.051 0.101 0.225 0.456 0.796 1.219 1.911 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.007 0.051 0.101 0.225 0.456 0.796 1.219 1.911 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.007 0.051 0.101 0.225 0.456 0.796 1.219 1.911 O.D. 0.007 0.051 0.101 0.225 0.456 0.796 1.219 1.911

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.