Mouse HRG ELISA Kit PicoKine®

HRG is a?glycoprotein?that in humans is encoded by the?HRG?gene.?The HRG protein is produced in the liver, and it could also be synthesized by?monocytes, ?macrophages, and?megakaryocytes.?It possesses a multi-domain?structure, which makes it capable of binding to numerous ligands and modulating various biological processes including?immunity, ?vascularization?and?coagulation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 273 302 289 294 14.61 4.9% Sample . 2 1100 1026 1014 1099 1059 39.97 3.7% Sample 3 4259 4189 4932 4609 4497 297.18 6.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 312 273 302 289 294 14.61 4.9% Sample 1 312 273 302 289 294 14.61 4.9% Sample . 2 1100 1026 1014 1099 1059 39.97 3.7% Sample . 2 1100 1026 1014 1099 1059 39.97 3.7% Sample 3 4259 4189 4932 4609 4497 297.18 6.6% Sample 3 4259 4189 4932 4609 4497 297.18 6.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1100 4259 314 1299 4301 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 312 1100 4259 314 1299 4301 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 312 1100 4259 314 1299 4301 Mean (pg/ml) 312 1100 4259 314 1299 4301 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 Standard deviation 12.79 49.5 264.06 17.27 75.34 326.88 CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6% CV (%) 4.1% 4.5% 6.2% 5.5% 5.8% 7.6%

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Binds heparin and heparin/glycosaminoglycans in a zinc-dependent manner. Binds heparan sulfate on the surface of liver, lung, kidney and heart endothelial cells. Binds to N-sulfated polysaccharide chains on the surface of liver endothelial cells. Inhibits rosette formation. Acts as an adapter protein and is implicated in regulating many processes such as immune complex and pathogen clearance, cell chemotaxis, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Acts as a regulator of the vascular endothelial growth factor (VEGF) signaling pathway; inhibits endothelial cell motility by reducing VEGF-induced complex formation between PXN/paxillin and ILK/integrin-linked protein kinase and by promoting inhibition of VEGF-induced tyrosine phosphorylation of focal adhesion kinases and alpha- actinins in endothelial cells. Also plays a role in the regulation of tumor angiogenesis and tumor immune surveillance. Normalizes tumor vessels and promotes antitumor immunity by polarizing tumor- associated macrophages, leading to decreased tumor growth and metastasis (By similarity) . Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.066 0.126 0.245 0.469 0.861 1.437 1.943 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.066 0.126 0.245 0.469 0.861 1.437 1.943 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.066 0.126 0.245 0.469 0.861 1.437 1.943 O.D. 0.021 0.066 0.126 0.245 0.469 0.861 1.437 1.943

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.