Mouse Serpin A1 ELISA Kit PicoKine®

Mouse Serpin A1 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Serpina1c in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

SERPINA1 is also known as PI, A1A or AAT. This gene is mapped to 14q32.1. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Reproducibility

Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample . 2 13987 13721 13461 14393 13890 344.60 2.4% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7% Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Lots Lot 1 (ng/ml) Lot 2 (ng/ml) Lot 3 (ng/ml) Lot 4 (ng/ml) Mean (ng/ml) Standard Deviation CV (%) Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample 1 2876 2791 2438 2616 2680 168.37 6.2% Sample . 2 13987 13721 13461 14393 13890 344.60 2.4% Sample . 2 13987 13721 13461 14393 13890 344.60 2.4% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7% Sample 3 37231 37794 38247 41965 38809 1857.18 4.7%

Synonyms

Serpina1c; Dom3; Dom6; Spi1-3; Spi1-6; Alpha-1-antitrypsin 1-3; Alpha-1 protease inhibitor 3; Alpha-1 protease inhibitor 6; Alpha-1-antitrypsin 1-6; Serine protease inhibitor 1-3; Serine protease inhibitor 1-6; Serine protease inhibitor A1c; Serpin A1c

Gene Name

Serpina1c

Gene ID

20702

UniProt

Q00896

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-K413

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Surface Molecules, Transcription, Transcription Factors, Transcription Factors/Regulators

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Mean (pg/ml) 2876 13987 37231 2911 14122 4269 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 Standard deviation 158.18 713.3 1600.9 177.57 932.05 320.18 CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5% CV (%) 5.5% 5.1% 4.3% 6.1% 6.6% 7.5%

Sensitivity

<10 pg/mL

Function

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin.

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse AAT

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Alpha-1-antitrypsin 1-3

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903 O.D. 0.023 0.076 0.172 0.261 0.433 0.745 1.236 1.903

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.