Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®

Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Serpinf2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Alpha-2-antiplasmin is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample . 2 13864 12175 13380 12105 12881 760.90 5.9%% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample 1 2710 2230 2706 2277 2480 227.86 9.1% Sample . 2 13864 12175 13380 12105 12881 760.90 5.9%% Sample . 2 13864 12175 13380 12105 12881 760.90 5.9%% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8% Sample 3 42376 42947 47567 46317 44801 2194.70 4.8%

Synonyms

Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2; Pli

Gene Name

Serpinf2

Gene ID

18816

UniProt

Q61247

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: N40-K491

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed by the liver and secreted in plasma.

Applications

ELISA

Field of Research

Angiogenesis, Cardiovascular, Cell Biology, Neurogenesis, Neurology Process, Neuroscience, Protease Inhibitors, Proteolysis/Ubiquitin, Sensory System, Visual System

Standard

100 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

1.56 ng/mL - 100 ng/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Mean (pg/ml) 2710 13864 42376 2900 14200 45464 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 Standard deviation 113.82 804.1 2712.06 130.5 965.6 3273.4 CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2% CV (%) 4.2% 5.8% 6.4% 4.5% 6.8% 7.2%

Sensitivity

<10 pg/mL

Function

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse GPC3

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

Serine (or cysteine) peptidase inhibitor, clade F, member 2

Subcellular Location

Secreted.

Sample Data

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086 O.D. 0.019 0.077 0.154 0.342 0.6252 1.049 1.508 2.086

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.