Mouse GPC2 ELISA Kit PicoKine®

Mouse GPC2 ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse Gpc2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Glypican 2 (GPC2), also known cerebroglycan, is a protein which in humans is encoded by the GPC2 gene.Cerebroglycan is a glycophosphatidylinositol-linked integral membrane heparan sulfate proteoglycan found in the developing nervous system. Cerebroglycan participates in cell adhesion and is thought to regulate the growth and guidance of axons. Cerebroglycan has especially high affinity for laminin-1

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 121 126 111 121 6.57 5.4% Sample . 2 499 417 473 425 453 33.89 7.4%% Sample 3 1231 1236 1124 1165 1189 46.83 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 128 121 126 111 121 6.57 5.4% Sample 1 128 121 126 111 121 6.57 5.4% Sample . 2 499 417 473 425 453 33.89 7.4%% Sample . 2 499 417 473 425 453 33.89 7.4%% Sample 3 1231 1236 1124 1165 1189 46.83 3.9% Sample 3 1231 1236 1124 1165 1189 46.83 3.9%

Synonyms

Glypican-2; Secreted glypican-2; Gpc2

Gene Name

Gpc2

Gene ID

71951

UniProt

Q8BKV1

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H22-S556

Capture Antibody

A monoclonal antibody from rat

Detection Antibody

A biotinylated polyclonal antibody from goat

Applications

ELISA

Field of Research

Cytoskeleton/ECM, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 499 1231 130 501 1456 Standard deviation 8.06 28.9 57.86 7.67 35.07 113.57 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 128 499 1231 130 501 1456 Standard deviation 8.06 28.9 57.86 7.67 35.07 113.57 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 128 499 1231 130 501 1456 Mean (pg/ml) 128 499 1231 130 501 1456 Standard deviation 8.06 28.9 57.86 7.67 35.07 113.57 Standard deviation 8.06 28.9 57.86 7.67 35.07 113.57 CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8% CV (%) 6.3% 5.8% 4.7% 5.9% 7.0% 7.8%

Sensitivity

<10 pg/mL

Function

Cell surface proteoglycan that bears heparan sulfate. May fulfill a function related to the motile behaviors of developing neurons (By similarity) . .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse SerpinF2

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Glypican 2 (cerebroglycan)

Subcellular Location

Cell membrane ; Lipid-anchor, GPI-anchor ; Extracellular side .

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.12 0.174 0.291 0.525 0.968 1.829 2.555 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.12 0.174 0.291 0.525 0.968 1.829 2.555 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.049 0.12 0.174 0.291 0.525 0.968 1.829 2.555 O.D. 0.049 0.12 0.174 0.291 0.525 0.968 1.829 2.555

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.