Human Haptoglobin ELISA Kit PicoKine®

Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family (haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans (HP and HPR) . There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 698 804 781 765 40.1 5.2% Sample 2 2764 2697 2705 2507 2668 96.62 3.6% Sample 3 9900 10211 10114 10441 10166 194.36 1.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 778 698 804 781 765 40.1 5.2% Sample 1 778 698 804 781 765 40.1 5.2% Sample 2 2764 2697 2705 2507 2668 96.62 3.6% Sample 2 2764 2697 2705 2507 2668 96.62 3.6% Sample 3 9900 10211 10114 10441 10166 194.36 1.9% Sample 3 9900 10211 10114 10441 10166 194.36 1.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 2764 9900 774 2895 9099 Standard deviation 37.34 110.56 712.8 39.47 167.91 718.82 CV (%) 4.8% 4% 7.2% 7.9% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 778 2764 9900 774 2895 9099 Standard deviation 37.34 110.56 712.8 39.47 167.91 718.82 CV (%) 4.8% 4% 7.2% 7.9% 5.8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 778 2764 9900 774 2895 9099 Mean (pg/ml) 778 2764 9900 774 2895 9099 Standard deviation 37.34 110.56 712.8 39.47 167.91 718.82 Standard deviation 37.34 110.56 712.8 39.47 167.91 718.82 CV (%) 4.8% 4% 7.2% 7.9% 5.8% 7.9% CV (%) 4.8% 4% 7.2% 7.9% 5.8% 7.9%

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.004 0.048 0.073 0.107 0.226 0.422 0.905 1.851 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.004 0.048 0.073 0.107 0.226 0.422 0.905 1.851 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.004 0.048 0.073 0.107 0.226 0.422 0.905 1.851 O.D. 0.004 0.048 0.073 0.107 0.226 0.422 0.905 1.851

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.