Rat PCSK9 ELISA Kit PicoKine®

PCSK9 is the 9th member of the proprotein convertase family of proteins that activate other proteins.PCSK9 has medical importance because it acts in lipoprotein homeostasis. PCSK9 is ubiquitously expressed in many tissues and cell types. PCSK9 binds to the receptor for low-density lipoprotein particles (LDL), which typically transport 3,000 to 6,000 fat molecules (including cholesterol) per particle, within extracellular fluid. The LDL receptor (LDLR), on liver and other cell membranes, binds and initiates ingestion of LDL-particles from extracellular fluid into cells, thus reducing LDL particle concentrations.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 143 162 163 152 10.55 6.9% Sample . 2 555 551 591 544 560 18.18 3.2%% Sample 3 1161 1116 1190 1010 1119 68.36 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 140 143 162 163 152 10.55 6.9% Sample 1 140 143 162 163 152 10.55 6.9% Sample . 2 555 551 591 544 560 18.18 3.2%% Sample . 2 555 551 591 544 560 18.18 3.2%% Sample 3 1161 1116 1190 1010 1119 68.36 6.1% Sample 3 1161 1116 1190 1010 1119 68.36 6.1%

Proprotein convertase subtilisin/kexin type 9; Neural apoptosis-regulated convertase 1; NARC-1; Proprotein convertase 9; PC9; Subtilisin/kexin-like protease PC9; Pcsk9; Narc1

Highly expressed in 12-day embryo. In the adult, strongly expressed in liver, small intestine, jejunum, and to a lesser extent in kidney, lung, spleen and thymus. Expression in the liver is up-regulated following partial hepatectomy.

Amino Acid Metabolism, Amino Acids, Cancer, Cardiovascular, Cell Biology, Cholesterol Metabolism, Intracellular, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Neural Stem Cells, Pathways and Processes, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 555 1161 145 563 1332 Standard deviation 6.58 30.53 68.5 9.28 37.72 99.9 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 140 555 1161 145 563 1332 Standard deviation 6.58 30.53 68.5 9.28 37.72 99.9 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 140 555 1161 145 563 1332 Mean (pg/ml) 140 555 1161 145 563 1332 Standard deviation 6.58 30.53 68.5 9.28 37.72 99.9 Standard deviation 6.58 30.53 68.5 9.28 37.72 99.9 CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5% CV (%) 4.7% 5.5% 5.9% 6.4% 6.7% 7.5%

Crucial player in the regulation of plasma cholesterol homeostasis. Binds to low-density lipid receptor family members: low density lipoprotein receptor (LDLR), very low density lipoprotein receptor (VLDLR), apolipoprotein E receptor (LRP1/APOER) and apolipoprotein receptor 2 (LRP8/APOER2), and promotes their degradation in intracellular acidic compartments. Acts via a non-proteolytic mechanism to enhance the degradation of the hepatic LDLR through a clathrin LDLRAP1/ARH-mediated pathway. May prevent the recycling of LDLR from endosomes to the cell surface or it to lysosomes for degradation. Can induce ubiquitination of LDLR leading to its subsequent degradation. Inhibits intracellular degradation of APOB via the autophagosome/lysosome pathway in a LDLR-independent manner. Involved in the disposal of non-acetylated intermediates of BACE1 in the early secretory pathway. Inhibits epithelial Na (+) channel (ENaC) -mediated Na (+) absorption by reducing ENaC surface expression primarily by increasing its proteasomal degradation. Regulates neuronal apoptosis via modulation of LRP8/APOER2 levels and related anti-apoptotic signaling pathways (By similarity) . .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cytoplasm . Secreted . Endosome . Lysosome . Cell surface . Endoplasmic reticulum . Golgi apparatus . Autocatalytic cleavage is required to transport it from the endoplasmic reticulum to the Golgi apparatus and for the secretion of the mature protein. Localizes to the endoplasmic reticulum in the absence of LDLR and colocalizes to the cell surface and to the endosomes/lysosomes in the presence of LDLR. The sorting to the cell surface and endosomes is required in order to fully promote LDLR degradation (By similarity) . .

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.073 0.141 0.201 0.312 0.537 0.854 1.451 2.071 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.073 0.141 0.201 0.312 0.537 0.854 1.451 2.071 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.073 0.141 0.201 0.312 0.537 0.854 1.451 2.071 O.D. 0.073 0.141 0.201 0.312 0.537 0.854 1.451 2.071

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.