Human PDGF-DD ELISA Kit PicoKine®

Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division.The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD) . The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 49 50 50 50 0.7 1.4% Sample . 2 222 183 220 190 203 17.44 8.5%% Sample 3 762 921 866 889 859 59.58 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 49 50 50 50 0.7 1.4% Sample 1 51 49 50 50 50 0.7 1.4% Sample . 2 222 183 220 190 203 17.44 8.5%% Sample . 2 222 183 220 190 203 17.44 8.5%% Sample 3 762 921 866 889 859 59.58 6.9% Sample 3 762 921 866 889 859 59.58 6.9%

Platelet-derived growth factor D; PDGF-D; Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form; IEGF; SCDGFB; MSTP036; UNQ1899/PRO4345

Expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen and colon. In the kidney, expressed by the visceral epithelial cells of the glomeruli. A widespread expression is also seen in the medial smooth muscle cells of arteries and arterioles, as well as in smooth muscle cells of vasa rectae in the medullary area. Expressed in the adventitial connective tissue surrounding the suprarenal artery. In chronic obstructive nephropathy, a persistent expression is seen in glomerular visceral epithelial cells and vascular smooth muscle cells, as well as de novo expression by periglomerular interstitial cells and by some neointimal cells of atherosclerotic vessels. Expression in normal prostate is seen preferentially in the mesenchyme of the gland while expression is increased and more profuse in prostate carcinoma. Expressed in many ovarian, lung, renal and brain cancer-derived cell lines.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 222 762 57 229 841 Standard deviation 2.81 9.1 43.4 3.53 14.89 61.39 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 222 762 57 229 841 Standard deviation 2.81 9.1 43.4 3.53 14.89 61.39 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 222 762 57 229 841 Mean (pg/ml) 51 222 762 57 229 841 Standard deviation 2.81 9.1 43.4 3.53 14.89 61.39 Standard deviation 2.81 9.1 43.4 3.53 14.89 61.39 CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3% CV (%) 5.5% 4.1% 5.7% 6.2% 6.5% 7.3%

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Plays an important role in wound healing. Induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. Can initiate events that lead to a mesangial proliferative glomerulonephritis, including influx of monocytes and macrophages and production of extracellular matrix (By similarity) .

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.177 0.268 0.435 0.691 1.083 1.922 2.867 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.177 0.268 0.435 0.691 1.083 1.922 2.867 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.177 0.268 0.435 0.691 1.083 1.922 2.867 O.D. 0.037 0.177 0.268 0.435 0.691 1.083 1.922 2.867

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.