Rat PDGF-BB ELISA Kit PicoKine®

PDGF-BB encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 107 108 107 107 0.50 0.4% Sample . 2 651 715 730 643 684 38.22 5.5%% Sample 3 1071 1047 1054 1145 1079 38.95 3.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 107 108 107 107 0.50 0.4% Sample 1 108 107 108 107 107 0.50 0.4% Sample . 2 651 715 730 643 684 38.22 5.5%% Sample . 2 651 715 730 643 684 38.22 5.5%% Sample 3 1071 1047 1054 1145 1079 38.95 3.6% Sample 3 1071 1047 1054 1145 1079 38.95 3.6%

Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Metabolism, Metabolism Processes, Organogenesis, Pathways and Processes, Response To Hypoxia, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 651 1071 121 714 1110 Standard deviation 4.43 33.85 58.9 7.6 52.84 86.58 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 108 651 1071 121 714 1110 Standard deviation 4.43 33.85 58.9 7.6 52.84 86.58 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 108 651 1071 121 714 1110 Mean (pg/ml) 108 651 1071 121 714 1110 Standard deviation 4.43 33.85 58.9 7.6 52.84 86.58 Standard deviation 4.43 33.85 58.9 7.6 52.84 86.58 CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8% CV (%) 4.1% 5.2% 5.5% 6.3% 7.4% 7.8%

Drastically down-regulated in response to PDGF-BB or cell injury, that promote smooth muscle cell proliferation and dedifferentiation. Seems to play a role in the development of the embryonic vascular system.

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.093 0.145 0.25 0.43 0.758 1.335 1.846 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.093 0.145 0.25 0.43 0.758 1.335 1.846 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.039 0.093 0.145 0.25 0.43 0.758 1.335 1.846 O.D. 0.039 0.093 0.145 0.25 0.43 0.758 1.335 1.846

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.