Rat FGFBP1 ELISA Kit PicoKine®

Rat FGFBP1 ELISA Kit PicoKine® (96 Tests) . Quantitate Rat Fgfbp1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. This gene encodes a secreted fibroblast growth factor carrier protein. The encoded protein plays a critical role in cell proliferation, differentiation and migration by binding to fibroblast growth factors and potentiating their biological effects on target cells. The encoded protein may also play a role in tumor growth as an angiogenic switch molecule, and expression of this gene has been associated with several types of cancer including pancreatic and colorectal adenocarcinoma. A pseudogene of this gene is also located on the short arm of chromosome 5.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 108 117 112 112 3.19 2.8% Sample . 2 566 629 674 611 620 38.71 6.2%% Sample 3 2662 2454 2765 2801 2670 135.00 5.0% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 112 108 117 112 112 3.19 2.8% Sample 1 112 108 117 112 112 3.19 2.8% Sample . 2 566 629 674 611 620 38.71 6.2%% Sample . 2 566 629 674 611 620 38.71 6.2%% Sample 3 2662 2454 2765 2801 2670 135.00 5.0% Sample 3 2662 2454 2765 2801 2670 135.00 5.0%

Synonyms

Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; Growth factor-binding protein 1; FGFBP-1; Fgfbp1

Gene Name

Fgfbp1

Gene ID

64535

UniProt

Q9QY10

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E21-C238

Capture Antibody

A monoclonal antibody from mouse

Detection Antibody

A biotinylated polyclonal antibody from goat

Tissue Specificity

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level) .

Applications

ELISA

Field of Research

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 566 2662 119 607 2774 Standard deviation 6.9 36.79 114.47 6.19 37.03 199.7 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 112 566 2662 119 607 2774 Standard deviation 6.9 36.79 114.47 6.19 37.03 199.7 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 112 566 2662 119 607 2774 Mean (pg/ml) 112 566 2662 119 607 2774 Standard deviation 6.9 36.79 114.47 6.19 37.03 199.7 Standard deviation 6.9 36.79 114.47 6.19 37.03 199.7 CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2% CV (%) 6.2% 6.5% 4.3% 5.2% 6.1% 7.2%

Sensitivity

<10 pg/mL

Function

Expressed in gut, eye, thymus, skin, lung, tongue, Purkinje cells and cerebral chorioid plexus (at protein level) .

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant rat FGFBP1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Fibroblast growth factor binding protein 1

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.07 0.125 0.191 0.304 0.535 0.918 1.522 2.153 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.07 0.125 0.191 0.304 0.535 0.918 1.522 2.153 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.07 0.125 0.191 0.304 0.535 0.918 1.522 2.153 O.D. 0.07 0.125 0.191 0.304 0.535 0.918 1.522 2.153

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.