Human ULBP1 ELISA Kit PicoKine®

UL16 binding protein 1 (ULBP1) is a cell surface glycoprotein encoded by ULBP1 gene located on the chromosome 6. ULBP1 is related to MHC class I molecules, but its gene maps outside the MHC locus. The protein encoded by this gene is a ligand of natural killer group 2, member D (NKG2D), an immune system-activating receptor on NK cells and T-cells. Binding of the encoded ligand to NKG2D leads to activation of several signal transduction pathways, including those of JAK2, STAT5, ERK and PI3K kinase/Akt. Also, in cytomegalovirus-infected cells, this ligand binds the UL16 glycoprotein and is prevented from activating the immune system. Three transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 93 109 107 103 6.22 6% Sample 2 781 789 758 879 801 46.02 5.7% Sample 3 2293 2172 2362 2030 2214 126.25 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 105 93 109 107 103 6.22 6% Sample 1 105 93 109 107 103 6.22 6% Sample 2 781 789 758 879 801 46.02 5.7% Sample 2 781 789 758 879 801 46.02 5.7% Sample 3 2293 2172 2362 2030 2214 126.25 5.7% Sample 3 2293 2172 2362 2030 2214 126.25 5.7%

Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 781 2716 108 716 2457 Standard deviation 5.77 58.57 162.8 7.77 60.86 194.1 CV (%) 5.5% 7.5% 7.1% 7.2% 8.5% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 105 781 2716 108 716 2457 Standard deviation 5.77 58.57 162.8 7.77 60.86 194.1 CV (%) 5.5% 7.5% 7.1% 7.2% 8.5% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 105 781 2716 108 716 2457 Mean (pg/ml) 105 781 2716 108 716 2457 Standard deviation 5.77 58.57 162.8 7.77 60.86 194.1 Standard deviation 5.77 58.57 162.8 7.77 60.86 194.1 CV (%) 5.5% 7.5% 7.1% 7.2% 8.5% 7.9% CV (%) 5.5% 7.5% 7.1% 7.2% 8.5% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.064 0.194 0.277 0.446 0.708 1.133 1.803 2.348 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.064 0.194 0.277 0.446 0.708 1.133 1.803 2.348 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.064 0.194 0.277 0.446 0.708 1.133 1.803 2.348 O.D. 0.064 0.194 0.277 0.446 0.708 1.133 1.803 2.348

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.